Lipid droplet-free nanovesicles extruded from stromal vascular fraction improve adipocyte regeneration in the centre of dermal graft.

ABSTRACT:

Background

The stromal vascular fraction (SVF) has been validated for enhancing tissue regeneration because of its concentration of multipotent cells and growth factors, and for mitigating inflammatory response due to its elimination of the majority of lipid droplets. However, it is difficult for fresh SVF to maintain bioactivity for a long period, and the loss of numerous tangible masses during preparation limits its application in repairing large volume defects. Here, we fabricated a self-assembly nanovesicle extruded from SVF (SVF-EVs) by mechanical shear and co-transplanted it with dermal microparticles to verify its potential for repairing large volume defects.

Methods

The SVF-EVs were prepared by removing the oil from adipose tissue followed by sequentially extruding S

SUBMITTER: Zeng Y 

PROVIDER: S-EPMC11881435 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature