Cas9-expressing cattle using the PiggyBac transposon all-in-one system.

ABSTRACT:

Background

Livestock, particularly cattle, are crucial for biotechnology fields, such as genetic breeding, infectious diseases, bioreactors, and specific disease models. However, genetic engineering in cattle has lagged due to long gestation periods, single embryo pregnancies, and high rearing costs. Additionally, the slow validation of germline transmission and the absence of germline-competent embryonic stem cells hinder progress. With the development of genome editing technologies like ZFN, TALEN, and CRISPR-Cas9, recent advancements have shown that Cas9-expressing pigs and chickens have been successfully produced. We hypothesize that generating CRISPR/Cas9-expressing cattle and their resources will provide a powerful resource for bovine genome editing, advancing our understandi

SUBMITTER: Kwon DH 

PROVIDER: S-EPMC11881473 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature