Xpert MTB/RIF Ultra-resistant and MTBDR<i>plus-</i>susceptible rifampicin results in people with tuberculosis: utility of FluoroType MTBDR and deep sequencing.

ABSTRACT: Xpert MTB/RIF Ultra (Ultra)-detected rifampicin-resistant tuberculosis (TB) is often programmatically confirmed using MTBDRplus. There are limited data on discordant results, including when re-tested using newer methods, like FluoroType MTBDR (FT-MTBDR) and targeted deep sequencing. MTBDRplus rifampicin-susceptible isolates from people with Ultra rifampicin-resistant sputum were identified from a South African programmatic laboratory. FT-MTBDR and single molecule-overlapping reads (SMOR; rpoB, inhA, katG) on isolate DNA were done (SMOR was used as a reference standard). Between 1 April 2021 and 30 September 2022, 8% (109/1347) of Ultra rifampicin-resistant specimens were MTBDRplus-susceptible. Of 89% (97/109) isolates with a sequenceable rpoB

SUBMITTER: Ghebrekristos Y 

PROVIDER: S-EPMC11881552 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature