Biodistribution and persistence of human umbilical cord-derived mesenchymal stem cells in NCG mice: a comparative study.

ABSTRACT:

Introduction

This study aims to investigate the biodistribution and persistence of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) in NCG mice post-intravenous injection, utilizing ⁸⁹Zr-PET/CT, bioluminescence imaging, multiplex immunohistochemistry (mIHC), and quantitative polymerase chain reaction (qPCR).

Methods

hUC-MSCs were labeled with ⁸⁹Zr-oxine (⁸⁹Zr-MSCs) or transduced with luciferase gene (Luc-MSCs). Real-time tracking of ⁸⁹Zr-MSCs lasted for 14-days followed by mIHC staining of hCD73. Real-time tracking of Luc-MSCs lasted for 7-days, followed by mIHC staining of hCD73 and human Alu-based qPCR. All methods adhered to ICH and other regulatory guidelines for development of cell-based drugs.

Results

A b

SUBMITTER: Liu G 

PROVIDER: S-EPMC11881841 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature