Impact of increasingly complex cell culture conditions on the proteome of human periodontal ligament stem cells.

ABSTRACT:

Aims

Human periodontal ligament stem cells (hPDLSCs) exhibit an enormous potential to regenerate periodontal tissue. However, their translatability to the clinical setting is constrained by technical difficulties in standardizing culture conditions. The aim was to assess complex culture conditions using a proteomic-based protocol to standardize multi-layer hPDLSC cultivation methodology.

Materials and methods

hPDLSC-derived constructs were created with varying biological complexity. The simplest constructs were monolayer sheets of hPDLSCs cultured with fetal bovine serum (FBS) or Plasma Rich in Growth Factors supernatant (PRGFsn). The most complex constructs were triple-layered cell structures cultured with PRGFsn, with or without PRGF fibrin membrane (mPRGF). Ultrastructure

SUBMITTER: Fullaondo A 

PROVIDER: S-EPMC11881847 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature