Characterizing heterologous protein burden in Komagataella phaffii.

ABSTRACT: Yeast is a widely utilized chassis for heterologous protein production, with Komagataella phaffii well-established as a prominent nonconventional yeast in this field. Despite its widespread recognition, there remains considerable potential to further optimize these cell factories to meet high production demands in a cost-effective and sustainable manner. Understanding the cellular response to the challenges of heterologous protein production can equip genetic engineers with crucial knowledge to develop enhanced strategies for constructing more efficient cell factories. In this study, we explore the molecular response of various K. phaffii strains that produce either the human insulin precursor or Mambalgin-1, examining changes in transcription and changes in intra- and extracellular protei

SUBMITTER: Kastberg LB 

PROVIDER: S-EPMC11881926 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature