Differential transcript and soluble factor patterns in macrophage/enterocyte-like monolayer co-cultures based on apical or basolateral LPS exposure.

ABSTRACT:

Background

The monolayer of intestinal epithelial cells (IECs) plays a crucial role in controlling intestinal homeostasis, also by its interaction with the immune system, via paracrine cytokine production, thus driving innate responses by tissue-resident immune cells. Here, using a co-culture model, we investigated the interactions between differentiated Caco-2 cells in monolayer and macrophages, by mimicking the cross-talk between enterocytes and immune cells during gastrointestinal (GI) tract inflammation.

Methods

Caco-2 mature monolayers grown on Transwell membranes were challenged with apical or basolateral LPS. After stimulations, the enterocyte-like monolayers were transferred in co-culture with THP-1 derived macrophages. The functional impact of treatments was evaluat

SUBMITTER: Mazzei A 

PROVIDER: S-EPMC11882427 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature