Super-resolution of nodal and paranodal disruption in anti-pan-neurofascin-associated autoimmune nodopathy.

ABSTRACT:

Introduction

In autoimmune nodopathies, autoantibodies target the nodes of Ranvier, impairing saltatory nerve conduction. Understanding the impact of autoantibody binding on protein assembly is crucial for gaining insights into the pathogenicity of different autoantibodies. We investigated nodal, paranodal, and cytoskeletal axonal proteins in teased fibers from a sural nerve biopsy of a patient with anti-pan-neurofascin autoantibodies. Conventional diagnostic tools, including fluorescence microscopy, often miss subtle alterations at the ultrastructural level.

Methods

We utilized direct stochastic optical reconstruction microscopy (dSTORM), a super-resolution fluorescence imaging technique, to assess the nanoscale architecture of nodal, paranodal, and cytoskeletal axonal prot

SUBMITTER: da Cruz Neris Geßner V 

PROVIDER: S-EPMC11882429 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature