Dissection of the inflammatory bowel disease transcriptome using genome-wide cDNA microarrays.

ABSTRACT:

Background

The differential pathophysiologic mechanisms that trigger and maintain the two forms of inflammatory bowel disease (IBD), Crohn disease (CD), and ulcerative colitis (UC) are only partially understood. cDNA microarrays can be used to decipher gene regulation events at a genome-wide level and to identify novel unknown genes that might be involved in perpetuating inflammatory disease progression.

Methods and findings

High-density cDNA microarrays representing 33,792 UniGene clusters were prepared. Biopsies were taken from the sigmoid colon of normal controls (n = 11), CD patients (n = 10) and UC patients (n = 10). 33P-radiolabeled cDNA from purified poly(A)+ RNA extracted from biopsies (unpooled) was hybridized to the arrays. We identified 500 and 272 transcripts dif

SUBMITTER: Costello CM 

PROVIDER: S-EPMC1188246 | biostudies-literature | 2005 Aug

REPOSITORIES: biostudies-literature