The correlation between sperm DNA methylation and DNA damage: a comparison of comet and TUNEL.

ABSTRACT:

Objective

To evaluate the relationship between sperm DNA methylation and DNA damage as determined by the comet or TUNEL assays.

Design

Retrospective research study.

Setting

University-based andrology and in vitro fertilization (IVF) laboratory.

Patients

Data came from 1,470 male partners (ages 18 and older) recruited from heterosexual couples (ages 18-45 years) seeking fertility treatments. These data were analyzed retrospectively from the Folic Acid and Zinc Supplementation Trial (FAZST) study.

Main outcome measures

Comet and TUNEL measures and associations with DNA methylation patterns.

Results

Comet and TUNEL values were correlated with one another across all samples (R ² = 0.34, P < 0.001); however, when assessi

SUBMITTER: Zimmerman H 

PROVIDER: S-EPMC11882583 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature