Defining bovine CpG epigenetic diversity by analyzing RRBS data from sperm of Montbeliarde and Holstein bulls.

ABSTRACT:

Introduction

Breed epigenetic diversity was recently detected in pig muscle and cattle blood, probably as a result of long-term selection for morphological adaptive and quantitative traits, persisting after embryo epigenetic reprogramming.

Methods

In our study, breed epigenetic diversity in the male germline from Holstein (H) and Montbéliarde (M) bulls was investigated using Reduced Representation Bisulfite Sequencing (RRBS) data publicly available at the NCBI database. Open-source Whole Genome Sequencing (WGS) data from H and M animals were used to estimate genetic diversity between the two breeds and, thus, correctly assess CpG positions with low frequencies or absence of SNPs.

Results

Sperm epigenetic diversity was studied in 356,635 SNP-free CpG positions, and a t

SUBMITTER: Capra E 

PROVIDER: S-EPMC11882585 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature