Effects of light exposure during IVF: transcriptomic analysis of murine embryos and embryo-derived EVs.

ABSTRACT:

Introduction

Light exposure of embryos during assisted reproduction affects embryo quality and implantation capacity in a wavelength dependent manner. We investigated the molecular mechanism of these light-induced changes through the comparative analysis of gene expression and regulatory miRNA profile of murine embryos cultured in dark environment and those exposed to white- or red filtered light. miRNA sequencing was used to assess the role of embryo-derived extracellular vesicles in the endometrium-embryo dialogue.

Methods

In vitro cultured mouse embryos at 3.5 days post coitum (dpc) were exposed to white or red filtered light. After 24 hours mRNA and miRNA content of the embryos as well as the miRNA content of embryo-derived extracellular vesicles were isolated and

SUBMITTER: Nagy B 

PROVIDER: S-EPMC11882875 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature