Molecular delineation and haplotype analysis of domain membrane protein (DMP) gene influencing in-vivo haploid induction in maize.

ABSTRACT: Domain membrane protein (DMP) gene is one of the key genes regulating in vivo haploid production in maize. Here, full-length sequence (931 bp) of DMP gene was sequenced in five mutant and five wild-type maize inbreds to study allelic variation. Two SNPs viz., T to C (SNP1) and G to A (SNP2) that distinguished wild-type and mutant- allele of DMP gene, converted methionine to threonine and alanine to threonine at positions 44 and 87, respectively. Two breeder-friendly PCR-based markers (DMP_SNP_TC and DMP_SNP_GA) specific to SNP1 and SNP2 were developed. These markers identified four haplotypes, viz., Hap-DMP-TG, Hap-DMP-CG, Hap-DMP-TA, and Hap-DMP-CA among a set of 48 diverse maize inbreds. Both SNP1 and SNP2 were present in DUF679_motif-2 and DUF679_motif-1, respectively and were conserved

SUBMITTER: Gain N 

PROVIDER: S-EPMC11882960 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature