Virtual library docking for cannabinoid-1 receptor agonists with reduced side effects.

ABSTRACT: Virtual library docking can reveal unexpected chemotypes that complement the structures of biological targets. Seeking agonists for the cannabinoid-1 receptor (CB1R), we dock 74 million tangible molecules and prioritize 46 high ranking ones for de novo synthesis and testing. Nine are active by radioligand competition, a 20% hit-rate. Structure-based optimization of one of the most potent of these (K_i = 0.7 µM) leads to '1350, a 0.95 nM ligand and a full CB1R agonist of G_i/o signaling. A cryo-EM structure of '1350 in complex with CB1R-G_i1 confirms its predicted docked pose. The lead agonist is strongly analgesic in male mice, with a 2-20-fold therapeutic window over hypolocomotion, sedation, and catalepsy and no observable conditioned place preference. These

SUBMITTER: Tummino TA 

PROVIDER: S-EPMC11882969 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature