Assessing the impact of airborne particulate pollution on human skin utilizing a novel human skin equivalent containing MUTZ-3-derived Langerhans cells.

ABSTRACT: Air pollution is an exogenous stressor known to have a detrimental impact on skin health through the induction of inflammation; however, the direct effect of topical pollution exposure is still being elucidated. Human skin equivalents (HSE) aim to reproduce in vitro the structure and function of the native skin tissue. However, HSEs typically lack skin-resident immune cells, which could play a key role in the inflammatory response induced by pollution exposure. We outline the development of a HSE-containing MUTZ-3-derived Langerhans cells (MUTZ-3-LCs), which show dendritic morphology and Langerhans cell marker expression. We demonstrated that HSE-containing MUTZ-3-LC have lower basal levels of proinflammatory cytokines, but topical stimulation with allergens and irritant compounds induced

SUBMITTER: Simpson A 

PROVIDER: S-EPMC11883110 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature