Spatio-temporal reconstruction of gene expression patterns in developing mice.

ABSTRACT: Understanding gene regulation in organism development is crucial in biology. Techniques like whole-mount in situ hybridization can reveal spatial gene expression in organs and tissues. However, capturing time-lapse movies of gene expression dynamics in embryos developing in utero, such as mice, remains technically challenging beyond the early stages. To address this, we present a method to integrate static snapshots of gene expression patterns across limb developmental stages, creating a continuous 2D reconstruction of gene expression patterns over time. This method interpolates small tissue regions over time to create smooth temporal trajectories of gene expression. We successfully applied it to a number of key genes in limb development, including Sox9, Hand2, and Bmp2. This approach enab

SUBMITTER: Avino-Esteban L 

PROVIDER: S-EPMC11883288 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature