Feeder-cell-free system for <i>ex vivo</i> production of natural killer cells from cord blood hematopoietic stem and progenitor cells.

ABSTRACT:

Introduction

Natural Killer (NK) cells hold significant promise as therapeutic agents in immuno-oncology due to their ability to target and eliminate cancerous and infected cells without causing graft-versus-host disease or cytokine release syndrome. However, the limited availability of robust, scalable methods for generating clinical-grade NK cells remains a limiting factor to broader clinical application.

Methods

Here we report the development of a novel feeder-cell-free culture system optimized for producing NK cells from cord blood-derived CD34⁺ hematopoietic stem and progenitor cells (HSPCs). Our method eliminates the need for feeder cells while achieving high yields of NK cells that exhibit unique marker expression and cytotoxic functions. Cord blood CD34

SUBMITTER: Martin Corredera M 

PROVIDER: S-EPMC11883473 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature