Solid-State Nanopore Sensors: Analyte Quantification by Event Frequency Analysis at High Voltages.

ABSTRACT: Solid state nanopores have emerged as an important electrical label-free single-molecule detection platform. While much effort has been spent on analyzing the current trace to determine size, shape and charge of the translocating species, a less studied aspect is the number of events and how this relates to analyte concentration. In this work we systematically investigate how the event frequency depends on voltage applied across the pore and show that this dependence can be utilized to determine target concentration. Importantly, this method does not require any calibration or any additional species added to the solution. Data analysis algorithms are introduced to accurately count events also for high voltages (up to 1 V). For double stranded DNA as model analyte, we find a linear relation

SUBMITTER: Jarlebark J 

PROVIDER: S-EPMC11883732 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature