Overhauser Dynamic Nuclear Polarization Enables Single Scan Benchtop ¹³C NMR Spectroscopy in Continuous-Flow.

ABSTRACT: Benchtop ¹³C NMR spectroscopy is highly attractive for reaction and process monitoring. However, insufficient premagnetization and low signal intensities largely prevent its application to flowing liquids. We show that hyperpolarization by Overhauser dynamic nuclear polarization (ODNP) can be used to overcome these problems, as ODNP operates on short time scales and results in strong ¹³C signal enhancements. Benchtop ¹³C NMR spectra with ODNP enhancement acquired in continuous-flow are reported here for the first time. We have investigated two ODNP approaches: direct ODNP, which transfers the polarization of unpaired electrons to ¹³C nuclei via direct hyperfine coupling, and indirect ODNP, in which the electron polarization is first transferred t

SUBMITTER: Phuong J 

PROVIDER: S-EPMC11883742 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature