Single-cell analysis of CD14⁺CD16⁺ monocytes identifies a subpopulation with an enhanced migratory and inflammatory phenotype.

ABSTRACT: Monocytes in the central nervous system (CNS) play a pivotal role in surveillance and homeostasis, and can exacerbate pathogenic processes during injury, infection, or inflammation. CD14⁺CD16⁺ monocytes exhibit diverse functions and contribute to neuroinflammatory diseases, including HIV-associated neurocognitive impairment (HIV-NCI). Analysis of human CD14⁺CD16⁺ monocytes matured in vitro by single-cell RNA sequencing identified a heterogenous population of nine clusters. Ingenuity pathway analysis of differentially expressed genes in each cluster identified increased migratory and inflammatory pathways for a group of clusters, which we termed Group 1 monocytes. Group 1 monocytes, distinguished by increased ALCAM, CD52, CD63, and SDC2, ex

SUBMITTER: Ruiz VY 

PROVIDER: S-EPMC11883828 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature