Molecular characterization and phylogenetic analysis of Crimean-Congo hemorrhagic fever virus, northwestern of Iran.

ABSTRACT:

Introduction

Crimean-Congo hemorrhagic fever (CCHF) is one of the most acute tick-transmitted zoonotic diseases. The purpose of this study was to determine the molecular characteristics and phylogenetic analysis of the CCHF virus based on the S-segment nucleocapsid gene in Ardabil Province, northwestern Iran.

Materials and methods

From November 2021 to May 2023, a total of 20 peripheral blood specimens were collected from suspected CCHF patients. Following RNA extraction, the partial nucleotide sequence of the S fragment was amplified (536 bp) using a one-step RT-PCR Kit. PCR products were successfully sequenced to perform phylogenetic analysis and haplotype distribution.

Results

The mean age of CCHF patients was 30.4 ± 10.45 years and all male patients had a history

SUBMITTER: Soozangar N 

PROVIDER: S-EPMC11883951 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature