Srebf2 mediates successful optic nerve axon regeneration via the mevalonate synthesis pathway.

ABSTRACT:

Background

Axon regeneration within the mammalian central nervous system is extremely limited. In optic neuropathy conditions like glaucoma, the inability of retinal ganglion cell (RGC) axons to regenerate is a major impediment to functional recovery. In contrast, adult teleost fish such as zebrafish can fully regenerate RGC axons enabling visual recovery from optic nerve (ON) injury making it an ideal model to probe the mechanisms of successful axon regeneration.

Methods

Laser Capture Microdissection followed by RNA-sequencing (LCM-seq) was used to identify genes and pathways differentially expressed in RGCs during ON regeneration. We validate these findings by in situ hybridization and qRT-PCR. Using loss- and gain-of-function experiments we demonstrate the necessity of sr

SUBMITTER: Hu M 

PROVIDER: S-EPMC11883989 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature