Establishment of a novel mouse model of colorectal cancer by orthotopic transplantation.

ABSTRACT:

Background

Colorectal cancer (CRC) represents a major malignancy that poses a significant threat to human health worldwide. The establishment of a reliable and pathologically relevant orthotopic model of CRC is crucial for gaining a deeper understanding of its molecular mechanisms and for developing more effective therapies. Nonetheless, the development of such models is fraught with challenges primarily owing to the technical complexities associated with the transplantation of CRC cells into the intestinal epithelium.

Methods

The luminal surface of the cecum was externalized to visualize the entire process involved in the transplantation of CRC cells into the cecal epithelium of BALB/c athymic nude mice. The cecal epithelium was mechanically removed, preserving the integrit

SUBMITTER: Chen C 

PROVIDER: S-EPMC11884030 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature