Transcriptional alterations of virulence factors in Leishmania major clinical isolates harboring Leishmania RNA virus 2 (LRV2).

ABSTRACT:

Background

Leishmaniasis is a parasitic disease caused by an intracellular protozoan, Leishmania. Various factors, including host immunity and the Leishmania species, influence the manifestation and severity of the disease. Recent investigations have shed light on the potentially significant role of Leishmania RNA virus (LRV) in the clinical prognosis of leishmaniasis. This study aims to investigate the influence of LRV2 + on various pathogenic genes of Leishmania.

Materials and methods

In this study, 35 Leishmania isolates were obtained from patients diagnosed with cutaneous leishmaniasis (CL). Leishmania species and the presence of LRV2 + were identified with the PCR-RFLP and semi-nested PCR methods, respectively. Additionally, the RNA expression levels of cysteine proteas

SUBMITTER: Sabeti S 

PROVIDER: S-EPMC11884114 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature