Pheno-Morphological Screening and Acoustic Sorting of 3D Multicellular Aggregates Using Drop Millifluidics.

ABSTRACT: Three-dimensional multicellular aggregates (MCAs) like organoids and spheroids have become essential tools to study the biological mechanisms involved in the progression of diseases. In cancer research, they are now widely used as in vitro models for drug testing. However, their analysis still relies on tedious manual procedures, which hinders their routine use in large-scale biological assays. Here, a novel drop millifluidic approach is introduced to screen and sort large populations containing over one thousand MCAs: ImOCAS (Image-based Organoid Cytometry and Acoustic Sorting). This system utilizes real-time image processing to detect pheno-morphological traits in MCAs. They are then encapsulated in millimetric drops, actuated on-demand using the acoustic radiation force. The performance

SUBMITTER: Rembotte L 

PROVIDER: S-EPMC11884609 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature