Identification of novel antiviral host factors by functional gene expression analysis using in vitro HBV infection assay systems.

ABSTRACT: To cure hepatitis B virus (HBV) infection, it is essential to elucidate the function of hepatocyte host factors in regulating the viral life cycle. Signaling and transcription activator of transcription (STAT)1 play important roles in immune responses, but STAT1-independent pathways have also been shown to have important biological reactivity. Using an in vitro HBV infection assay system, the current study aimed to investigate the STAT1-independent host factors that contribute to the control of viral infection by comprehensive functional screening. The in vitro HBV infection system was established using primary human hepatocytes (PXB cells) infected with HBV derived from a plasmid containing the 1.3-mer HBV genome. Comprehensive functional studies were performed using small interfering RNA

SUBMITTER: Nosaka T 

PROVIDER: S-EPMC11884705 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature