Antifreeze proteins produced by Antarctic yeast from the genus Glaciozyma as cryoprotectants in food storage.

ABSTRACT: Synthesis of antifreeze proteins (AFPs) is one of the adaptations of psychrophilic yeast to live in cold environments. AFPs demonstrate thermal hysteresis (TH) activity and inhibit the recrystallization of ice (IRI) during periodic temperature fluctuations. In this study, the Antarctic yeast strain 186, identified as Glaciozyma martinii, was found to synthesize an extracellular, glycosylated ~27 kDa ice-binding protein (GmAFP) exhibiting IRI activity. It is the first evidence of AFP secretion by the psychrophilic yeast Glaciozyma martinii. To scale up protein production, a synthetic gene from a closely related cold-adapted species, Glaciozyma antarctica, was expressed in Pichia pastoris GS115 strain. The recombinant 26.57 kD protein (GaAFP) displayed IRI activity and a cryoprotective effec

SUBMITTER: Majewska E 

PROVIDER: S-EPMC11884722 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature