Elucidation of <i>Callistemon lanceolatus</i>-derived natural compounds in STAT 3 pathway against human cancer cells: <i>in silico</i> and <i>in vitro</i> studies.

ABSTRACT:

Introduction

Many human tumours have hyperactive signal transducer and activator of transcription 3 (STAT3, positioning it as a prime target for natural compounds with anticancer properties. This study investigated three small-molecule STAT3 inhibitors derived from Callistemon lanceolatus: cyanidin-3,5-diglucoside, kaempferol-3-o-β-d-galactopyranoside, and quercetin-3-o-(2″-o-galloyl)-β-d-galactopyranoside.

Material and methods

The compounds were explored through virtual screening and molecular dynamics (MD) simulations to understand the intracellular processing of activated STAT3. The biological effects of these STAT3 inhibitors on human cancer cells were assessed via simulation. Further, in-vitro studies were performed to exhibit the anti-cancer role of compo

SUBMITTER: Zafar M 

PROVIDER: S-EPMC11885254 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature