<i>Staphylococcus aureus</i> utilizes vimentin to internalize human keratinocytes.

ABSTRACT:

Introduction

Vimentin is an intermediate filamentous cytoskeletal protein involved in cell migration, adhesion, and division. Recent studies have demonstrated that several bacteria and viruses interact with vimentin to facilitate entry and trafficking within eukaryotic cells. However, the relationship between Staphylococcus aureus and vimentin remains unclear.

Methods

In the current study, we elucidated vimentin expression mechanism in human keratinocytes infected with S. aureus using Western blot (WB), Flow cytometry, Immunofluorescence (IF) staining, utilizing neutralizing antibodies, and small interference (si) RNA, and a vimentin overexpression vector. The physical interaction between vimentin and S. aureus was shown by IF on cell surface, intra- and

SUBMITTER: Jang K 

PROVIDER: S-EPMC11885264 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature