Discovery of Novel Protein-Coding and Long Non-coding Transcripts in Distinct Regions of the Human Brain.

ABSTRACT: Recent improvements in the accuracy of long-read sequencing (LRS) technologies have expanded the scope for novel transcriptional isoform discovery. Additionally, these advancements have improved the precision of transcript quantification, enabling a more accurate reconstruction of complex splicing patterns and transcriptomes. Thus, this project aims to take advantage of these analytical developments for the discovery and analysis of RNA isoforms in the human brain. A set of novel transcript isoforms was compiled using three bioinformatic tools, quantifying their expression across eight replicates of the cerebellar hemisphere, five replicates of the frontal cortex, and six replicates of the putamen. By taking a subset of the novel isoforms consistent across all discovery methods, a set of 1

SUBMITTER: Santucci K 

PROVIDER: S-EPMC11885362 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature