Alpha-globin gene cluster haplotypes and D1S80, D17S5, and TPO VNTR polymorphisms among four ethnic populations from lower northeastern Thailand.

ABSTRACT: Laos, Khmer, Kui, and Yer ethnic populations had a high prevalence of α⁺-thalassemia, but the genetic background remains poorly understood. This study examined genetic variation at the α-globin gene cluster and three VNTR loci (D1S80, D17S5, and TPO) in these ethnic populations. For α-globin haplotype analysis, 110 subjects with normal α-globin and 232 subjects with α⁺-thalassemia were selected to analyze six polymorphic sites using the PCR-RFLP technique. For VNTR loci analysis, 447 subjects were examined for D1S80, D17S5, and TPO allele frequencies using a PCR-based method. The results of this study revealed that the most frequent haplotypes found in Laos, including (framework 1; + M - + - 0) linked to (-α^3.7) and (framework 3; - S - + + -) related to (α<

SUBMITTER: Singsanan S 

PROVIDER: S-EPMC11885464 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature