SART3 promotes homologous recombination repair by stimulating DNA-RNA hybrids removal and DNA end resection.

ABSTRACT: DNA-RNA hybrids triggered by double-strand breaks (DSBs) are crucial intermediates during DSB repair, and their timely resolution requires numbers of RNA helicases, including DEAD box 1 (DDX1). However, how these helicases are recruited to DSB-induced hybrids in time remains largely unclear. Here, we revealed that squamous cell carcinoma antigen recognized by T cells 3 (SART3) promotes DDX1 binding to DNA-RNA hybrids at DSBs for optimal homologous recombination (HR) repair. SART3 itself associates with DNA-RNA hybrids and PAR chains and accumulates at DSBs in both PARylation- and DNA-RNA hybrids-dependent fashion. SART3 also associates with DDX1 and is necessary for DDX1 enrichment at DSBs. The defective SART3-DDX1 association observed in cells expressing the cancer-associated variant SART

SUBMITTER: Fu H 

PROVIDER: S-EPMC11885473 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature