Prostaglandin F2 receptor negative regulator as a potential target for chimeric antigen receptor-T cell therapy for glioblastoma.

ABSTRACT:

Background

Chimeric antigen receptor (CAR)-T cell therapy targeting novel glioblastoma (GBM)-specific cell surface antigens is a promising approach. However, transcriptome analyses have revealed few GBM-specific target antigens.

Methods

A library of monoclonal antibodies (mAbs) against tumor cell lines derived from patients with GBM was generated. mAbs reacting with tumor cells in resected tissues from patients with GBM but not with nonmalignant human brain cells were detected. The antigens that were recognized were identified through expression cloning. CAR-T cells derived from a candidate mAb were generated, and their functionality was tested in vitro and in vivo.

Results

Approximately 3,200 clones were established. Among them, 5E17 reacted with tumor cells in six o

SUBMITTER: Kuroda H 

PROVIDER: S-EPMC11885767 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature