Exploring ultraviolet and nitrous acid-induced mutagenesis of Rhizopus oligosporus for extracellular inulinase production under solid-state culture.

ABSTRACT: This study details the synthesis and optimization of extracellular inulinase through solid-state fermentation using improved strain of Rhizopus oligosporus. The wild-type was procured from IIB culture bank and subsequently enhanced through UV-radiation and Nitrous acid treatments. The resulting mutant strain was subjected to further optimization for heightened enzyme production via solid-state fermentation. The substrate, pressmud (20 g), and moisture content (20 mL molasses) were carefully selected. Key parameters such as inoculum size, incubation time, and inulin concentration were optimized to maximize enzyme yield. Mutation procedures involved optimizing spore suspension, UV-radiation exposure duration, and the distance of culture plate from UV rays. Nitrous acid treatment, followed by

SUBMITTER: Khalid SW 

PROVIDER: S-EPMC11885772 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature