Energy filtering enables macromolecular MicroED data at sub-atomic resolution.

ABSTRACT: High-resolution information is important for accurate structure modeling but is challenging to attain in macromolecular crystallography due to the rapid fading of diffracted intensities at increasing resolution. While direct electron detection essentially eliminates the read-out noise during MicroED data collection, other sources of noise remain and limit the measurement of faint high-resolution reflections. Inelastic scattering significantly contributes to noise, raising background levels and broadening diffraction peaks. We demonstrate a substantial improvement in signal-to-noise ratio by using energy filtering to remove inelastically scattered electrons. This strategy results in sub-atomic resolution MicroED data from proteinase K crystals, enabling the visualization of detailed structu

SUBMITTER: Clabbers MTB 

PROVIDER: S-EPMC11885823 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature