Impact of Short-Chain Fatty Acids on Glucose, Fatty Acid and Leucine Metabolism in Primary Human Myotubes.

ABSTRACT:

Introduction

Short-chain fatty acids (SCFAs) are small molecule metabolites mainly produced during microbial fermentation of dietary fibre in the gut and have been shown to have a beneficial impact on human health. The aim of this study was to evaluate the effect of SCFAs on human skeletal muscle energy metabolism.

Methods

Primary human myotubes were analysed for glucose and fatty acid (oleic acid) metabolism, as well as insulin sensitivity and protein synthesis in the presence or absence of SCFAs.

Results

The most pronounced effects of SCFAs were observed on ¹⁴C-oleic acid uptake and oxidation, as well as ¹⁴C-leucine uptake and protein synthesis, following butyrate treatment. Butyrate increased ¹⁴C-leucine accumulation twofold, potent

SUBMITTER: Tingstad RH 

PROVIDER: S-EPMC11885951 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature