EIL (ethylene-insensitive 3-like) transcription factors in apple affect both ethylene- and cold response-dependent fruit ripening.

ABSTRACT: EIN3/EIL (ethylene-insensitive 3/EIN3-like) transcription factors are positive downstream transcriptional regulators of ethylene signalling. In apple (Malus × domestica), a small family of MdEIL genes was identified, with four expressed in fruit. Transgenic lines were generated to manipulate MdEIL1 expression, and fruits were sampled at harvest maturity and after cold treatment. Their fruit ripening behaviour was compared with control lines and contrasted to a ACC OXIDASE 1 antisense line (ACO1as) which produced no ripening associated ethylene. Two transgenic lines showed strong co-suppression of MdEIL1-4 expression as well as reduced ethylene production, softening and aroma production, while one overexpressing line showed enhanced ripening. Key genes involved in ethylene biosynthesis and

SUBMITTER: Feng J 

PROVIDER: S-EPMC11886506 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature