Site-Selective Approaches to Attain Fluorescent Human Insulin Conjugates: Balancing the Site of Labeling and the <i>In Vivo</i> Activity.

ABSTRACT: Fluorescent insulin is commonly used for a range of detection and imaging purposes. Achieving site-selective insulin labeling affords superior labeling yield while retaining its biological activity. Insulin labeling is usually achieved using commercial kits with minimal emphasis on the site and degree of labeling. To bridge this gap, this work highlights the essential parameters concerning the development of fluorescent insulin and reflects them on the biological activity of insulin in vivo. To this end, monolabeled insulin at the N-terminal of A chain (Gly^A1-N-FITC-insulin) was prepared using the minimal equivalents of fluorescein isothiocyanate (FITC) dye. In our hands, temperature and pH control were the main parameters affecting the reaction yield, with no dilabeled i

SUBMITTER: Alkhawaja B 

PROVIDER: S-EPMC11886657 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature