Papain-Catalyzed Hydrolysis of <i>N</i> _α-Benzoyl-arginine-<i>p</i>-nitroanilide in an Aqueous-Organic Medium.

ABSTRACT: Enzyme-based biosensors have emerged as an effective alternative, providing simplicity, high sensitivity, and the capability to detect multiple residues. However, despite their widespread use, limited studies have examined how organic solvents inhibit these sensors. This study investigates the enzymatic reactions and structure of the selected model enzyme, papain, a protease derived from Carica papaya, in the presence of various organic solvents. Enzyme activity was monitored through the hydrolysis of N_α-benzoyl-arginine-p-nitroanilide (BAPNA), with the resulting yellow product, p-nitroaniline, measured at a wavelength of 430 nm. The experiments incorporated a 10% (v/v) concentration of dimethyl sulfoxide (DMSO) to ensure the solubility of BAPNA. Results sho

SUBMITTER: Sirirak J 

PROVIDER: S-EPMC11886738 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature