PARP2 Catalytic Activity Is Allosterically Stimulated by Binding to the c-KIT1 G-Quadruplex.

ABSTRACT: PARP2, along with PARP1, is involved in the maintenance of the genomic stability. PARP2 catalyzes the formation of poly(ADP-ribose) to recruit repair proteins at the site of DNA breaks. Single-strand (SSB) and double-strand (DSB) DNA breaks are bona fide stimulators of PARP2 catalytic activity. However, the regulation of PARP2 catalytic activity by G-quadruplex DNA structures, abundant in regulatory and promoter regions of proto-oncogene and telomeres, has not been studied. Here, we report that PARP2 binds to the proto-oncogene-specific parallel G-quadruplex, c-KIT1-G4. c-KIT1-G4 stimulates the catalytic activity of PARP2. However, the c-KIT1-G4-dependent stimulation of PARP2 catalytic activity is 2-fold lesser than 5'P-SSB DNA, a cognate stimulator of PARP2 catalytic activity. The N-termi

SUBMITTER: Singh Hanuman D 

PROVIDER: S-EPMC11886757 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature