Ultra-sensitive detection of transposon insertions across multiple families by transposable element display sequencing.

ABSTRACT:

Background

Mobilization of transposable elements (TEs) can generate large effect mutations. However, due to the difficulty of detecting new TE insertions in genomes and the typically rare occurrence of transposition, the actual rate, distribution, and population dynamics of new insertions remain largely unexplored.

Results

We present a TE display sequencing approach that leverages target amplification of TE extremities to detect non-reference TE insertions with high specificity and sensitivity, enabling the detection of insertions at frequencies as low as 1 in 250,000 within a DNA sample. Moreover, this method allows the simultaneous detection of insertions for distinct TE families, including both retrotransposons and DNA transposons, enhancing its versatility and cost-effec

SUBMITTER: Vendrell-Mir P 

PROVIDER: S-EPMC11887134 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature