The CGA1-SNAT regulatory module potentially contributes to cytokinin-mediated melatonin biosynthesis and drought tolerance in wheat.

ABSTRACT:

Background

Melatonin plays a pivotal role in alleviating abiotic stresses, yet its biosynthesis regulation in crops, particularly wheat, remains unclear. This study explores regulatory components of melatonin biosynthesis under drought stress using bioinformatic, physiochemical, and molecular approaches in contrasting wheat genotypes.

Results

Bioinformatic analysis identified SNAT, a key melatonin biosynthesis gene, and 88 transcription factors (TFs) from 26 families as potential regulators. The regulatory network for SNAT highlighted CYTOKININ-RESPONSIVE GATA FACTOR 1 (CGA1) as a significant TF. Under drought stress, contrasting wheat genotypes exhibited distinct CGA1-SNAT module expression, melatonin and cytokinin levels, photosynthetic activity, and oxidative damage. Cyto

SUBMITTER: Shamloo-Dashtpagerdi R 

PROVIDER: S-EPMC11887191 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature