Enhancing Bacillus cereus antibacterial ability through improved cofactor supply.

ABSTRACT: Bacillus cereus 0-9 is a biocontrol microorganism that antagonizes Gram-positive bacteria and pathogenic fungi, such as Staphylococcus aureus and Gaeumannomyces graminis, through the secretion of antimicrobial peptides. However, its low antibacterial activity limits its biocontrol application. In this study, a significant enhancement in antibacterial activity against S. aureus was achieved by overexpressing glucose dehydrogenase from Bacillus subtilis (BsGDH) in B. cereus 0-9, expanding the activity from 6.98 to 11.59 U/mL, representing a 66% improvement. To further improve its biocontrol capability, we aimed to improve the catalytic efficiency of BsGDH by screening 11 low-conserved residues in the protein's second-shell via conservation analysis and molecular docking. Following three roun

SUBMITTER: Xu Y 

PROVIDER: S-EPMC11887302 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature