PD-1^IR2 promotes tumor evasion via deregulating CD8⁺ T cell function.

ABSTRACT:

Background

The programmed cell death 1 (PD-1) is an immune checkpoint that mediates immune evasion of tumors. Alternative splicing (AS) such as intron retention (IR) plays a crucial role in the immune-related gene processing and its function. However, it is not clear whether PDCD1 encoding PD-1 exists as an IR splicing isoform and what underlying function of such isoform plays in tumor evasion.

Methods

An AS isoform of human PDCD1, characterized by the second IR and named PD-1^IR2, was identified by reverse transcription-PCR (RT-PCR) and Sanger sequencing. The expression profile of PD1^IR2 was assessed by quantitative RT-PCR and flow cytometry, while its function was evaluated through immune cell proliferation, cytokine interleukin 2 secreti

SUBMITTER: Zang H 

PROVIDER: S-EPMC11887316 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature