Rewiring Escherichia coli to transform formate into methyl groups.

ABSTRACT:

Background

Biotechnological applications are steadily growing and have become an important tool to reinvent the synthesis of chemicals and pharmaceuticals for lower dependence on fossil resources. In order to sustain this progression, new feedstocks for biotechnological hosts have to be explored. One-carbon (C₁-)compounds, including formate, derived from CO₂ or organic waste are accessible in large quantities with renewable energy, making them promising candidates. Previous studies showed that introducing the formate assimilation machinery from Methylorubrum extorquens into Escherichia coli allows assimilation of formate through the C₁-tetrahydrofolate (C₁-H₄F) metabolism. Applying this route for formate assimilation, we here i

SUBMITTER: Mohr MKF 

PROVIDER: S-EPMC11887345 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature