Effects of hypoxia stress on the milk synthesis in bovine mammary epithelial cells.

ABSTRACT:

Background

Milk synthesis is an energy-intensive process influenced by oxygen availability. This study investigates how hypoxia affects milk synthesis in BMECs, focusing on key genes involved in lactation and energy metabolism.

Methods

BMECs were cultured in a normoxic environment and then transferred to a hypoxia chamber with 1% O₂ for specified durations. The study evaluated cellular responses through various molecular experiments and RNA sequencing. Small interfering RNA was employed to knock down HIF-1α to investigate whether the lactation-related phenotype alteration depends on HIF-1α.

Results

Hypoxia disrupted milk protein production by reducing mTOR/P70S6K/4EBP1 signaling and downregulating genes critical for amino acid transport and protein synthesi

SUBMITTER: Jin Y 

PROVIDER: S-EPMC11887346 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature