Novel UHPLC-(+ESI)MS/MS Method for Determining Amygdalin, Prunasin and Total Cyanide in Almond Kernels and Hulls (<i>Prunus dulcis</i>).

ABSTRACT: Almonds contain cyanogenic glycosides (CNGs), prunasin and amygdalin, which generate hydrogen cyanide upon hydrolysis. Different extraction and analytical methods are currently used to measure CNGs or cyanide (CN), necessitating distinct samples and can lead to inconsistent or incomparable results. To address this, we describe a method that uses ultrasonic-assisted sample extraction. Amygdalin and prunasin are measured directly in the extract, whereas CN is measured in the extract after derivatization with cysteine ethyl ester to form a cyano-S-ethyl-O-cysteine (CNCysEt) conjugate. The amygdalin, prunasin, and CNCysEt are quantified using the same UHPLC-(+ESI)MS/MS method. This new approach measured total CN in ten common almond kernel and hull varieties. The limit of quantit

SUBMITTER: Doria E 

PROVIDER: S-EPMC11887422 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature