Interplay of Surface Charge and Pore Characteristics in the Immobilization of Lactate Oxidase on Bulk Nanoporous Gold Electrodes.

ABSTRACT: Immobilization of enzymes on (nano)porous metal carriers provides the foundation for an advanced design of bioelectrodes suitable for catalysis and sensing. However, interactions upon adsorption are still poorly understood, and so the efficient coupling of the enzymes to the electrode surface remains one of the major challenges. Here, we present a comprehensive study of the immobilization behavior of Aerococcus viridans l-lactate oxidase (LOx) on nanoporous gold (npAu) in dependence of electrode modification with differently charged self-assembled monolayers (SAMs). The highest activity (up to 14 U/g) and electrocatalytic response (sensitivity of 3.9 μA mM^-1) were observed for a sulfonate-terminated SAM. This is contrary to enzyme behavior on conventional polymer carriers

SUBMITTER: Novak LM 

PROVIDER: S-EPMC11887427 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature