Molecular Determinants of Optical Modulation in ssDNA-Carbon Nanotube Biosensors.

ABSTRACT: Most traditional optical biosensors operate through molecular recognition, where ligand binding causes conformational changes that lead to optical perturbations in the emitting motif. Optical sensors developed from single-stranded DNA-functionalized single-walled carbon nanotubes (ssDNA-SWCNTs) have started to make useful contributions to biological research. However, the mechanisms underlying their function have remained poorly understood. In this study, we combine experimental and computational approaches to show that ligand binding alone is not sufficient for optical modulation in this class of synthetic biosensors. Instead, the optical response that occurs after ligand binding is highly dependent on the chemical properties of the ligands, resembling mechanisms seen in activity-based bi

SUBMITTER: Krasley AT 

PROVIDER: S-EPMC11887485 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature