Comparing eDNA and Transect Methods for Aquatic Biodiversity Assessment in Lakes and Ponds.

ABSTRACT: Biodiversity monitoring increasingly relies on molecular methods such as eDNA metabarcoding. However, sound applications have so far been only established for a limited number of taxonomic groups. More information on the strengths and weaknesses of eDNA methods, especially for poorly covered groups, is essential for practical applications to achieve the highest possible reliability. We compared amphibian and Odonata data from eDNA metabarcoding and traditional transect walks on N = 56 plots in 38 water bodies distributed over six extraction sites for building materials in Northwest Germany. The traditional amphibian assessment included visual encounters, dip netting and acoustic detection, while Odonata were assessed through exuviae. In total, both methods detected 8 out of 11 amphibian sp

SUBMITTER: Schwesig K 

PROVIDER: S-EPMC11887607 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature